This study aims to develop and validate the Teachers' Spiritual Leadership Questionnaire (TSLQ) to assess teachers' spiritual leadership from students' perspectives. Grounded in the principles of spiritual leadership—Vision, Hope/Faith, and Altruistic Love—the TSLQ explores how teachers inspire, influence, and guide students toward both academic and holistic development. The study addresses the lack of assessment tools in educational settings and introduces a structured validation process to ensure the instrument's accuracy and reliability. The questionnaire was developed and refined through several stages using expert input, literature review, and statistical validation methods. A total of 402 students participated, and their responses were analyzed using factor analysis to examine the tool's structure and effectiveness. Findings confirmed that the TSLQ is valid and reliable, with strong alignment between items and the three key dimensions of spiritual leadership. The results supported the model's overall strength, indicating that the questionnaire effectively captures the intended constructs. The study concludes that the TSLQ is a sound instrument for understanding spiritual leadership in the classroom and can help educators and researchers better assess its impact on students' academic and personal growth. Further research is recommended to test the tool in different cultural and educational settings and to explore additional dimensions of spiritual leadership. This new tool offers valuable insights for enhancing teaching practices and promoting a supportive and values-driven learning environment.